nucleic acid detection qrt pcr

Predesigned TaqMan Assays

Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by our TaqMan Assay performance guarantee. You can also order your assay with special off-catalog manufacturing modifications using our Specialty service or leverage Thermo Fisher’s manufacturing infrastructure with our GMP oligos optionsor OEM and contract manufacturing services.

With TaqMan Assays, gold standard performance is always guaranteed

Applied Biosystems TaqMan Assays have long been considered the gold standard in quantitative genomic and proteomic analysis, providing high specificity, reproducibility, sensitivity, and unsurpassed content. We have invested years of research and verification to offer you the industry’s largest portfolio of application-specific, predesigned assays ready to work right out of the box. TaqMan Assays are developed using a robust probe/primer design pipeline ensuring that every assay delivers results you can trust.

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.

nucleic acid detection qrt pcr
       nucleic acid detection qrt pcr

Introduction

The phrase “Polymerase chain reaction” (PCR) was first used more than 30 years ago in a paper describing a novel enzymatic amplification of DNA (Saiki et al., 1985). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that comprise steps of DNA denaturation, primer annealing, and extension, have not been changed since 1985. The invention of PCR has greatly boosted research in various areas of biology and this technology has significantly contributed to the current level of human knowledge in many spheres of research.

985). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that comprise steps of DNA denaturation, primer annealing, and extension, have not been changed since 1985. The invention of PCR has greatly boosted research in various areas of biology and this technology has significantly contributed to the current level of human knowledge in many spheres of research.

One-Step BrightGreen qRT-PCR-ROX

G471-R 100 rxn (20 ul/rxn)
EUR 158

One-Step TaqProbe qRT-PCR-ROX

G493-P 100 rxn (20 ul/rxn)
EUR 158

One-Step TaqProbe qRT-PCR-iCycler

G493-PC 100 rxn (20 ul/rxn)
EUR 158

MDR Mycobacterium tuberculosis (Rifampin and Isoniazid multiplex detection) PCR kit

PCR-H652-48D 50T
EUR 425.8
Description: An conventional PCR kit for detection of MDR Mycobacterium tuberculosis (Rifampin and Isoniazid multiplex detection)

MDR Mycobacterium tuberculosis (Rifampin and Isoniazid multiplex detection) PCR kit

PCR-H652-96D 100T
EUR 521.5
Description: An conventional PCR kit for detection of MDR Mycobacterium tuberculosis (Rifampin and Isoniazid multiplex detection)

Cellular Nucleic Acid Binding Protein

20-abx261023
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 885

One-Step BrightGreen qRT-PCR-Low ROX

G471-LR 100 rxn (20 ul/rxn)
EUR 158

One-Step BrightGreen qRT-PCR-No Dye

G471-S 100 rxn (20 ul/rxn)
EUR 158

One-Step TaqProbe qRT-PCR-Low ROX

G493-PL 100 rxn (20 ul/rxn)
EUR 158

One-Step TaqProbe qRT-PCR-No Dye

G493-PS 100 rxn (20 ul/rxn)
EUR 158

ExCellenCT One-Step BrightGreen qRT-PCR-iCycler

G917-iC 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step BrightGreen qRT-PCR-ROX

G917-R 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step TaqProbe qRT-PCR-ROX

G918-P 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step TaqProbe qRT-PCR-iCycler

G918-PC 25 Preps 100 x 20 ul reactions
EUR 177

PCR Mycoplasma Detection Kit

abx098883-100rxns 100 rxns
EUR 537

PCR Mycoplasma Detection Kit

abx298017-100rxns 100 rxns
EUR 314

PCR-Salmonella Detection Kit

K1447-96
EUR 620

PCR-STEC Detection Kit

K1452-96
EUR 784

PCR-Campylobacter Detection Kit

K1453-96
EUR 784

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Mycoplasma PCR Detection Kit

K1476-100 100 Rxns
EUR 298

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 801

Green One-Step qRT-PCR SuperMix (GC Rich)

20-abx09803920ulSystems
  • EUR 592.00
  • EUR 1024.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix (GC Rich)

20-abx09804120ulSystems
  • EUR 495.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX

G917-LR 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step BrightGreen qRT-PCR-No Dye

G917-S 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX

G918-PL 25 Preps 100 x 20 ul reactions
EUR 177

ExCellenCT One-Step TaqProbe qRT-PCR-No Dye

G918-PS 25 Preps 100 x 20 ul reactions
EUR 177

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 408
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 594
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

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