nucleic acid detection qrt pcr

Predesigned TaqMan Assays

Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by our TaqMan Assay performance guarantee. You can also order your assay with special off-catalog manufacturing modifications using our Specialty service or leverage Thermo Fisher’s manufacturing infrastructure with our GMP oligos optionsor OEM and contract manufacturing services.

With TaqMan Assays, gold standard performance is always guaranteed

Applied Biosystems TaqMan Assays have long been considered the gold standard in quantitative genomic and proteomic analysis, providing high specificity, reproducibility, sensitivity, and unsurpassed content. We have invested years of research and verification to offer you the industry’s largest portfolio of application-specific, predesigned assays ready to work right out of the box. TaqMan Assays are developed using a robust probe/primer design pipeline ensuring that every assay delivers results you can trust.

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.

nucleic acid detection qrt pcr
       nucleic acid detection qrt pcr

Introduction

The phrase “Polymerase chain reaction” (PCR) was first used more than 30 years ago in a paper describing a novel enzymatic amplification of DNA (Saiki et al., 1985). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that comprise steps of DNA denaturation, primer annealing, and extension, have not been changed since 1985. The invention of PCR has greatly boosted research in various areas of biology and this technology has significantly contributed to the current level of human knowledge in many spheres of research.

985). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that comprise steps of DNA denaturation, primer annealing, and extension, have not been changed since 1985. The invention of PCR has greatly boosted research in various areas of biology and this technology has significantly contributed to the current level of human knowledge in many spheres of research.

PCR Mycoplasma Detection Kit

G238 100 Reactions
EUR 195
Description:

The Mycoplasma PCR Detection Kit offers high specificity and sensitivity to minimize false positives while ensuring coverage over 200 strains of Mycoplasmas for quick and reliable routine screening of cell cultures less than 2 hours. Mycoplasmas are highly undesirable, easily acquired and notorious for an elusive onset of infection. It can alter the infected cells at a molecular level leading to visible changes in cell morphology and growth characteristics. Timely detection of Mycoplasmas in cell cultures is recommended in order to deter wide-spread contamination and save on the costly efforts of elimination. The MasterMix contains gel loading dye, making it convenient to load for gel electrophoresis.

PCR Mycoplasma Detection Kit

abx298017-100rxns 100 rxns
EUR 376.8

PCR Mycoplasma Detection Kit

abx098883-100rxns 100 rxns
EUR 644.4

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 204.33
Description: N/A

Mycoplasma PCR Detection Kit

K1476-100 100 Rxns
EUR 357.6

PCR Mycoplasma Detection Kit

abx298017-100g 100 µg Ask for price

PCR Mycoplasma Detection Kit

abx298017-20g 20 µg
EUR 437.5

PCR Mycoplasma Detection Kit

abx298017-50g 50 µg Ask for price

Salmonella Detection Kit (PCR)

abx092289-100l 100 µl
EUR 937.5

Salmonella Detection Kit (PCR)

abx092289-1ml 1 ml Ask for price

Salmonella Detection Kit (PCR)

abx092289-200l 200 µl
EUR 1775

Mycoplasma PCR Detection Kit

BMC1040-1000T 1000 T
EUR 199

Mycoplasma PCR Detection Kit

BMC1040-200T 200 T
EUR 59

Mycoplasma PCR Detection Kit

BMC1040-each each Ask for price

PCR-Salmonella Detection Kit

K1447-96 each
EUR 744

PCR-Campylobacter Detection Kit

K1453-96 each
EUR 940.8

genesig Real-time PCR detection kit for L.acidophilus

Z-Path-L-acidophilus 150 tests
EUR 808
Description: L.acidophilus

Peanut Allergen Detection Kit (PCR)

abx092288-100l 100 µl
EUR 775

Peanut Allergen Detection Kit (PCR)

abx092288-1ml 1 ml Ask for price

Peanut Allergen Detection Kit (PCR)

abx092288-200l 200 µl
EUR 1437.5

PCR-Legionella spp Detection Kit

K1450-96 each
EUR 777.6

genesig Std Real-time PCR detection kit for L.acidophilus

Z-Path-L-acidophilus-std 150 tests
EUR 602
Description: L.acidophilus

PCR detection kit for Monkeypox Virus

DA1430 24 test/kit
EUR 129.6
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.

MALARIA DETECTION KIT-REAL TIME PCR

MBPCR092-10R 1 unit
EUR 46.44
Description: MALARIA DETECTION KIT-REAL TIME PCR

PCR-Salmonella-Listeria Detection Kit

K1448-96 each
EUR 940.8

PCR-Listeria monocytogenes Detection Kit

K1449-96 each
EUR 777.6

PCR-Legionella spp Plus Detection Kit

K1451-96 each
EUR 966

BisulPlus Loci 5mC & 5hmC Detection PCR Kit

P-1067-48 2x 24 reactions
EUR 577.5

BisulPlus Loci 5mC & 5hmC Detection PCR Kit

P-1067 2x 24 Reactions
EUR 542

Aspergillus Niger Taqman PCR Detection, 100 reactions

TBS42023 100 tests
EUR 198

Nucleic Acid

N15180 25G
EUR 158.05

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