nucleic acid detection qrt pcr

Predesigned TaqMan Assays

Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. No additional design, optimization, or melt curve analysis needed. Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves—not to mention they’re backed by our TaqMan Assay performance guarantee. You can also order your assay with special off-catalog manufacturing modifications using our Specialty service or leverage Thermo Fisher’s manufacturing infrastructure with our GMP oligos optionsor OEM and contract manufacturing services.

With TaqMan Assays, gold standard performance is always guaranteed

Applied Biosystems TaqMan Assays have long been considered the gold standard in quantitative genomic and proteomic analysis, providing high specificity, reproducibility, sensitivity, and unsurpassed content. We have invested years of research and verification to offer you the industry’s largest portfolio of application-specific, predesigned assays ready to work right out of the box. TaqMan Assays are developed using a robust probe/primer design pipeline ensuring that every assay delivers results you can trust.

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.

nucleic acid detection qrt pcr
       nucleic acid detection qrt pcr

Introduction

The phrase “Polymerase chain reaction” (PCR) was first used more than 30 years ago in a paper describing a novel enzymatic amplification of DNA (Saiki et al., 1985). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that comprise steps of DNA denaturation, primer annealing, and extension, have not been changed since 1985. The invention of PCR has greatly boosted research in various areas of biology and this technology has significantly contributed to the current level of human knowledge in many spheres of research.

985). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that comprise steps of DNA denaturation, primer annealing, and extension, have not been changed since 1985. The invention of PCR has greatly boosted research in various areas of biology and this technology has significantly contributed to the current level of human knowledge in many spheres of research.

PCR-Campylobacter Detection Kit

K1453-96 each
EUR 940.8

PCR-Legionella spp Detection Kit

K1450-96 each
EUR 777.6

PCR detection kit for Monkeypox Virus

DA1430 24 test/kit
EUR 129.6
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.

PCR-Salmonella-Listeria Detection Kit

K1448-96 each
EUR 940.8

PCR-Listeria monocytogenes Detection Kit

K1449-96 each
EUR 777.6

Nucleic Acid Instrument

Smart-32 1 unit/set Ask for price

Nucleic Acid Instrument

Swift-96 1 unit/set Ask for price

PCR-Legionella spp Plus Detection Kit

K1451-96 each
EUR 966

BisulPlus Loci 5mC & 5hmC Detection PCR Kit

P-1067-48 2x 24 reactions
EUR 577.5

Ecl Direct Nucleic Acid

RPN3000 EACH
EUR 1122

Ecl Direct Nucleic Acid

RPN3001 EACH
EUR 1899.6

Ecl Direct Nucleic Acid

RPN3005 EACH
EUR 834

Nucleic Acid Removal Kit

NAR911 22 ml
EUR 105.7
Description: This product includes 22 ml of NAR reagent 1 and 22 ml of Reagent 2. It is sufficient for treatment of 200 ml of cell lysate or protein solution.

Coronavirus (SARS-CoV-2) PCR Detection Kit

K1460 100 Rxns
EUR 684
Description: Kit for detection of SARS-CoV-2 in respiratory specimens using Real-Time (RT-PCR).

Coronavirus (SARS-CoV-2) PCR Detection Kit

K1460-100 100 Rxns
EUR 661.2

HemogloBind™ Nucleic Acid

HN250-20 20 preps
EUR 436.8
Description: DNA/RNA Enrichment Kit

HemogloBind™ Nucleic Acid

HN250-200 200 preps
EUR 1216.8
Description: DNA/RNA Enrichment Kit

Monkeypox Virus Detection Kit (RT-PCR)

SYNT-RTPCR-24 24 test/kit Ask for price

rno-mir-1 RT-PCR Detection Kit

20-abx096332
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-1 RT-PCR Detection Kit

20-abx096024
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-7 RT-PCR Detection Kit

20-abx096025
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9 RT-PCR Detection Kit

20-abx096026
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9* RT-PCR Detection Kit

20-abx096027
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7a RT-PCR Detection Kit

20-abx096015
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7b RT-PCR Detection Kit

20-abx096016
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7c RT-PCR Detection Kit

20-abx096017
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7e RT-PCR Detection Kit

20-abx096019
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7f RT-PCR Detection Kit

20-abx096020
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7g RT-PCR Detection Kit

20-abx096021
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7i RT-PCR Detection Kit

20-abx096022
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7f RT-PCR Detection Kit

20-abx096023
  • EUR 644.40
  • EUR 878.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

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