heat labile udg vazyme

Introduction

The Vazyme VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina  is specially designed for the preparation of stranded transcriptome libraries for next generation sequencing (NGS) platforms of Illumina. The initial template is prepared with 0.1 µg-1 µg of total RNA of
human, mouse, or rat. Compared with traditional transcriptome library preparation kits, this kit enables the depletion of ribosomal RNA
(rRNA), including both cytoplasmic (28S, 18S, 5.8S, and5S) and mitochondrial (16S and 12S) rRNA, leaving mRNA and other non-coding
RNA behind. This kit is suitable for subsequent non-coding RNA analysis such as lncRNA. Degraded RNA (i.e. FFPE RNA) could also be
used to prepare the library with this kit. In addition, this kit enables the insertion of dUTP during the 2nd strand synthesis of cDNA. The
double strand cDNA are digested by uracil-DNA glycosylase (UDG) to remove the second strand before sequencing. As a result, only
information from the 1st strand cDNA is preserved. In addition to standard transcriptome information, strand-specific (i.e. from sense or
anti-sense DNA) information can also be obtained from NGS data analysis.

heat labile udg vazyme
                                                  heat labile udg vazyme

Applications

Requirements for Starting Materials: 0.1 µg-1 µg total RNA of human, mouse. Degraded RNA (i.e. FFPE RNA) could also be used to
prepare the library with this kit.
Information of Transcripts: : This kit is applicable to stranded mRNA or non-coding RNA (with the exception of rRNA) related analysis
with RNA-seq, including gene expression analysis, single nucleotide variation calling, alternative splicing / fusion detection, target
transcriptome analysis, and target genes prediction and functional analysis.
If conventional mRNA of animal, plant, or fungal is under consideration, please use the VAHTS mRNA-seq V2 Library Prep Kit for Illumina
(Vazyme, #NR601) for library construction. If stranded mRNA of animal, plant, or fungal is under consideration, please use the VAHTS
Stranded RNA-seq Library Prep Kit for Illumina (Vazyme, #NR602) for library construction.

Additional Materials Required

DNA Clean Beads: VAHTS DNA Clean Beads (Vazyme,#N411) or Agencourt MPure XP Beads (Beckman Coulter, #A63880, #A63881,
#A63882).
RNA Clean Beads: VAHTS RNA Clean Beads (Vazyme, #N412) or Agencourt RNAClean  XP Beads(Beckman Coulter, #A63987).
RNA Analysis:Agilent RNA 6000 Pico Kit (Agilent, #5067-1513).
Library Analysis: Agilent DNA 1000 Kit (Agilent, #5067-1504).
Adapters: VAHTS RNA Adapters Set 1 – Set 2 for Illumina (Vazyme, #N803, #N804), or VAHTS RNA Adapters Set 3 – Set 6 for Illumina
(Vazyme, #N809, #N810, #N811, #N812).
Other Materials: Fresh Ethanol (80%), Nuclease-free Water, Nuclease-free PCR tubes, Low absorption EP tubes, Agilent 2100 Bioanalyzer, Thermocyler (PCR instrument) ,Magnetic stand.

VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina is specially designed for the preparation of transcriptome libraries for next generation
sequencing (NGS) platforms of Illumina. The kit is universal and suitable for RNA library construction of RNA that have been obtained by
Poly(A)-based mRNA enrichment or rRNA depletion . The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library
construction for non-stranded transcriptome or stranded transcriptome analysis.
This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process
of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with
lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained
after size selections with magnetic beads.
01/ Introduction
All Components should be stored at -30℃ ~ -15℃ , transported at -20℃ ~ 0℃.
VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina is suitable for RNA library construction of RNA that have been enriched by Poly(A) (for RNA
with good integrity from eukaryotes such as animals, plants and fungi) or rRNA depletion.As the content of mRNA in total RNA of different samples
varies greatly, enough total RNA need to be inputted to make sure the sufficient mRNA for library construction. The amount of input-RNA is related to
the mRNA enrichment module:
VAHTS mRNA Capture Beads (Vazyme #N401): 0.05μg – 4 μg;
Ribo-off rRNA Depletion Kit (H/R/M) (Vazyme #N406): 0.05μg – 1 μg;
Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 1 μg – 5 μg;
Ribo-off rRNA Depletion Kit (Plant) (Vazyme #N409): 1 μg – 5 μg;
It is recommended to use an Agilent 2100 Bioanalyzer to analyze the integrity of total RNA. mRNA enrichment using VAHTS® mRNA Capture Beads
(Vazyme #N401) needs high quality RNA samples (RIN ≥ 7). Degraded total RNA used for library construction will lead to 3’ bias in RNA-seq. For RNA
samples with RIN value < 7, rRNA removal can be performed using the Ribo-off method (Vazyme #N406/407/409).
Main fields of RNA-related analysis:
◇gene expression analysis
◇single nucleotide variation calling
◇alternative splicing detection
◇gene fusion detection
◇target transcriptome analysis
04/ Applications
02/ Components
03/ Storage

2 × Vazyme LAmp Master Mix

P311-03 15 ml
EUR 401

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-01 125 U
EUR 121

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-02 500 U
EUR 175

2 × Vazyme LAmp Master Mix (Dye Plus)

P312-01 1 ml
EUR 138

2 × Vazyme LAmp Master Mix (Dye Plus)

P312-02 5 ml
EUR 212

2 × Vazyme LAmp Master Mix (Dye Plus)

P312-03 15 ml
EUR 401

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d1 125 U
EUR 123

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d2 500 U
EUR 178

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d1 125 U
EUR 123

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d2 500 U
EUR 178

Heat Labile Enterotoxin antibody

10-1016 500 ug
EUR 349
Description: Mouse monoclonal Heat Labile Enterotoxin antibody

Heat Labile Enterotoxin antibody

10-1017 1 mg
EUR 349
Description: Mouse monoclonal Heat Labile Enterotoxin antibody

Heat Labile Enterotoxin antibody

10-1018 500 ug
EUR 349
Description: Mouse monoclonal Heat Labile Enterotoxin antibody

Heat Labile Enterotoxin antibody

10-1019 500 ug
EUR 349
Description: Mouse monoclonal Heat Labile Enterotoxin antibody

Heat-labile enterotoxin B chain Antibody

20-abx110561
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

UDG antibody

70R-50531 100 ul
EUR 244
Description: Purified Polyclonal UDG antibody

UDG Antibody

20-abx008589
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

UDG Plasmid

PVT7010 2 ug
EUR 512

Escherichia coli Heat-Labile Enterotoxin A/B (E. coli Labile Toxin) Antibody

abx411328-02mg 0.2 mg
EUR 565

Heat-labile enterotoxin B chain Antibody (Biotin)

20-abx106290
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Heat-labile enterotoxin B chain Antibody (FITC)

20-abx107704
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Heat-labile enterotoxin B chain Antibody (HRP)

20-abx109122
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Escherichia coli Heat-Labile Enterotoxin A (E. coli Labile Toxin A / eltA) Antibody

abx411329-02mg 0.2 mg
EUR 565

Escherichia coli Heat-labile enterotoxin B chain (eltB)

1-CSB-EP315643ENL
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Heat-labile enterotoxin B chain(eltB) expressed in E.coli

Escherichia coli Heat-labile enterotoxin A chain (eltA)

1-CSB-EP356922ENL
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Heat-labile enterotoxin A chain(eltA) expressed in E.coli

Escherichia coli Heat-labile enterotoxin A chain (eltA)

1-CSB-EP356922ENLa3
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Heat-labile enterotoxin A chain(eltA) expressed in E.coli

Human heat- labile enterotoxin B subunit ELISA Kit

ELA-E0491h 96 Tests
EUR 824

Anti-UDG Antibody

A01672 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for UDG Antibody (UNG) detection.tested for WB in Human, Mouse, Rat.

UDG Blocking Peptide

20-abx063406
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

UDG Polyclonal Antibody

ABP52674-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of UDG from Human, Mouse, Rat. This UDG antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human UDG at AA range: 160-240

UDG Polyclonal Antibody

ABP52674-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of UDG from Human, Mouse, Rat. This UDG antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human UDG at AA range: 160-240

UDG Polyclonal Antibody

ABP52674-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of UDG from Human, Mouse, Rat. This UDG antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human UDG at AA range: 160-240

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