nu q h3 1 elisa assay ruo

Nu.Q H3.1

Determine levels of H3.1-containing, circulating cell-free nucleosomes

Nucleosomes: The Next Generation of Cancer Biomarkers

A nucleosome, composed of histone proteins and the DNA wrapped around them, is the core unit of chromatin. The affect nucleosomes have on chromatin structure and gene regulation is dependent upon the identity of the histones that make up the nucleosome, as well as any modifications to these histone proteins. With a plethora of histone protein variants and post-translational modifications that can occur on their “histone tails”, there are a vast number of possibilities when it comes to the exact identiity of a nucleosome and its function. Recently, some of these variants and modifications have been found to associate with cancers. Furthermore, just the total amount of nucleosomes detected can also indicate a disease state or correlate with efficacy of therapy.

Recently, “liquid biopsies”, or blood draws from which you can examine biomarkers, have taken hold as an easy, less invasive method to monitor disease state and treatment efficacy. Nucleosomes, histones, and DNA can be shed into the blood during cancer due to high cellular turnover or in response to chemotherapy. Free-floating DNA (cfDNA and ctDNA) is an attractive biomarker, but has shown to be less stable than nucleosomes. Additionally, next generation sequencing of DNA is cumbersome and costly compared to a high-throughput ELISA assay to detect intact nucleosomes. Nucleosome-specific detection assays represent a new generation of biomarker tests: easy, fast, cost-effective, and compatible with stable proteins in liquid biopsy samples.

Histone H3.1

Active Motif’s Nu.Q H3.1 Assay Kit is designed for the detection of levels of Histone H3.1-containing, circulating cell-free nucleosomes (cf-nucleosomes) in human serum in a high-throughput format.  Histone H3.1, or the canonical form of Histone H3, is deposited during DNA replication and possibly also during repair. Study of H3.1 has identified cancer-associated mutations and differing affinities for the histone acetyltransferase HAT1. To examine H3-containing nucleosome for all variants, use our Nu.Q H3 Assay. For added convenience and a more quantitative interpretation of results, the Nu.Q H3.1 Assay Kit also includes a recombinant nucleosome protein for use as a reference standard curve. For complete details, click the Nu.Q Method tab below.

nu q h3 1 elisa assay ruo
nu q h3 1 elisa assay ruo

Why use Nu.Q H3.1?

  • Sensitivity: Detect circulating nucleosomes in as little as 10 µl of serum or 20 µl of plasma
  • Specificity: Nucleosome epitope specific antibody enables detection of only intact nucleosomes
  • Quantification: Recombinant nucleosomes enable relative quantification of levels of circulating H3.1 nucleosomes
  • Convenience: Colorimetric assay in a simple 96-stripwell format for high and low throughput
  • Fast: Results can be obtained in 5 hoursA nucleosome, composed of histone proteins and the DNA wrapped around them, is the core unit of chromatin. The affect nucleosomes have on chromatin structure and gene regulation is dependent upon the identity of the histones that make up the nucleosome, as well as any modifications to these histone proteins. With a plethora of histone protein variants and post-translational modifications that can occur on their “histone tails”, there are a vast number of possibilities when it comes to the exact identiity of a nucleosome and its function. Recently, some of these variants and modifications have been found to associate with cancers. Furthermore, just the total amount of nucleosomes detected can also indicate a disease state or correlate with efficacy of therapy.

    Recently, “liquid biopsies”, or blood draws from which you can examine biomarkers, have taken hold as an easy, less invasive method to monitor disease state and treatment efficacy. Nucleosomes, histones, and DNA can be shed into the blood during cancer due to high cellular turnover or in response to chemotherapy. Free-floating DNA (cfDNA and ctDNA) is an attractive biomarker, but has shown to be less stable than nucleosomes. Additionally, next generation sequencing of DNA is cumbersome and costly compared to a high-throughput ELISA assay to detect intact nucleosomes. Nucleosome-specific detection assays represent a new generation of biomarker tests: easy, fast, cost-effective, and compatible with stable proteins in liquid biopsy samples.

Tertiapin-Q

B5090-1 1 mg
EUR 547

Cortisol in Saliva Elisa Kit (RUO)

55R-RE52061 96 wells
EUR 358
Description: ELISA kit for detection of Free Cortisol in Saliva in the research laboratory

NU 7026

HY-15719 10mM/1mL
EUR 126

NU 9056

A4492-10 10 mg
EUR 284
Description: Selective KAT5 (Tip60) histone acetyltransferase inhibitor (IC50 values are 100 ?M for KAT5, p300, pCAF and GCN5, respectively). Inhibits protein acetylation in prostate cancer cell lines and blocks DNA damage response.

NU 1025

A4530-25 25 mg
EUR 440
Description: Novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP). Ki and IC50 values are 48 and 400 nM respectively.

NU 1025

A4530-5 5 mg
EUR 180
Description: Novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP). Ki and IC50 values are 48 and 400 nM respectively.

NU 1025

A4530-50 50 mg
EUR 788
Description: Novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP). Ki and IC50 values are 48 and 400 nM respectively.

NU 7026

A8649-10 10 mg
EUR 137
Description: NU 7026 is a selective inhibitor of  DNA-PK with IC50 value of 0.23 ?M [1].

NU 7026

A8649-50 50 mg
EUR 421
Description: NU 7026 is a selective inhibitor of  DNA-PK with IC50 value of 0.23 ?M [1].

Nu 6027

A1986-10 10 mg
EUR 176
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

Nu 6027

A1986-5 5 mg
EUR 129
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

Nu 6027

A1986-5.1 10 mM (in 1mL DMSO)
EUR 131
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

Nu 6027

A1986-50 50 mg
EUR 547
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

NU-7441

B1117-25
EUR 544

NU-7441

B1117-5
EUR 175

NU-7026

B2522-25
EUR 544

NU-7026

B2522-5
EUR 175

NU 1025

2351-5
EUR 262

Q-VD-OPh

1170-1
EUR 234

anti-PKC nu (Protein kinase C nu)

LF-PA0073 100 ul
EUR 334
Description: Rabbit polyclonal to PKC nu (Protein kinase C nu)

Q-VD(OMe)-OPh

A8165-1 1 mg
EUR 177
Description: Q-VD-OPh (quinolyl-valyl-O-methylaspartyl-[-2,6-difluorophenoxy]-methyl ketone) is a broad spectrum caspase inhibitor,  provides a cost effective, non toxic, and highly specific means of apoptotic inhibition and provides new insight into the design of new inhibitors.

Q-VD-OPh hydrate

A1901-1 1 mg
EUR 177
Description: The broad spectrum caspase inhibitor, QVD-OPh, provides a cost effective, non toxic, and highly speci?c means of apoptotic inhibition and provides new insight into the design of new inhibitors1.

EZSolution? Q-VD-OPh

1173-1
EUR 207

EZSolution? Q-DEVD-OPh

1178-1
EUR 272

Protein Kinase C Nu Type (PKC nu) Antibody

abx033122-400ul 400 ul
EUR 523

Protein Kinase C Nu Type (PKC nu) Antibody

abx033122-80l 80 µl
EUR 286

Protein Kinase C Nu Type (PKC nu) Antibody

abx033123-400ul 400 ul
EUR 523

Protein Kinase C Nu Type (PKC nu) Antibody

abx033123-80l 80 µl
EUR 286

PKC nu antibody

20R-2919 100 ul
EUR 393
Description: Rabbit polyclonal PKC nu antibody

PKC nu protein

30R-2860 5 ug
EUR 503
Description: Purified recombinant Human PKC nu protein

EZSolution? NU-7441

B1875-5
EUR 185

EpiQuik Global Histone H3 Acetylation Assay Kit

P-4008 96 Assays
EUR 788.05
Description: kits suitable for this type of research

EZSolution? Q-VD-OPh, Negative Control

1174-1
EUR 207

Caspase-3 Inhibitor Q-DEVD-OPh

1175-1
EUR 272

Caspase-8 Inhibitor, Q-IETD-OPh

1176-1
EUR 305

Caspase-9 Inhibitor, Q-LEHD-OPh

1177-1
EUR 294

Polyclonal PKC nu Antibody

APR14376G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PKC nu . This antibody is tested and proven to work in the following applications:

Anti-PKC nu (4G7)

YF-MA17975 100 ug
EUR 363
Description: Mouse monoclonal to PKC nu

Human TDP2 Assay Kit

TG1005-1 100 assays
EUR 537

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