nu q h3 1 elisa assay ruo

Nu.Q H3.1

Determine levels of H3.1-containing, circulating cell-free nucleosomes

Nucleosomes: The Next Generation of Cancer Biomarkers

A nucleosome, composed of histone proteins and the DNA wrapped around them, is the core unit of chromatin. The affect nucleosomes have on chromatin structure and gene regulation is dependent upon the identity of the histones that make up the nucleosome, as well as any modifications to these histone proteins. With a plethora of histone protein variants and post-translational modifications that can occur on their “histone tails”, there are a vast number of possibilities when it comes to the exact identiity of a nucleosome and its function. Recently, some of these variants and modifications have been found to associate with cancers. Furthermore, just the total amount of nucleosomes detected can also indicate a disease state or correlate with efficacy of therapy.

Recently, “liquid biopsies”, or blood draws from which you can examine biomarkers, have taken hold as an easy, less invasive method to monitor disease state and treatment efficacy. Nucleosomes, histones, and DNA can be shed into the blood during cancer due to high cellular turnover or in response to chemotherapy. Free-floating DNA (cfDNA and ctDNA) is an attractive biomarker, but has shown to be less stable than nucleosomes. Additionally, next generation sequencing of DNA is cumbersome and costly compared to a high-throughput ELISA assay to detect intact nucleosomes. Nucleosome-specific detection assays represent a new generation of biomarker tests: easy, fast, cost-effective, and compatible with stable proteins in liquid biopsy samples.

Histone H3.1

Active Motif’s Nu.Q H3.1 Assay Kit is designed for the detection of levels of Histone H3.1-containing, circulating cell-free nucleosomes (cf-nucleosomes) in human serum in a high-throughput format.  Histone H3.1, or the canonical form of Histone H3, is deposited during DNA replication and possibly also during repair. Study of H3.1 has identified cancer-associated mutations and differing affinities for the histone acetyltransferase HAT1. To examine H3-containing nucleosome for all variants, use our Nu.Q H3 Assay. For added convenience and a more quantitative interpretation of results, the Nu.Q H3.1 Assay Kit also includes a recombinant nucleosome protein for use as a reference standard curve. For complete details, click the Nu.Q Method tab below.

nu q h3 1 elisa assay ruo
nu q h3 1 elisa assay ruo

Why use Nu.Q H3.1?

  • Sensitivity: Detect circulating nucleosomes in as little as 10 µl of serum or 20 µl of plasma
  • Specificity: Nucleosome epitope specific antibody enables detection of only intact nucleosomes
  • Quantification: Recombinant nucleosomes enable relative quantification of levels of circulating H3.1 nucleosomes
  • Convenience: Colorimetric assay in a simple 96-stripwell format for high and low throughput
  • Fast: Results can be obtained in 5 hoursA nucleosome, composed of histone proteins and the DNA wrapped around them, is the core unit of chromatin. The affect nucleosomes have on chromatin structure and gene regulation is dependent upon the identity of the histones that make up the nucleosome, as well as any modifications to these histone proteins. With a plethora of histone protein variants and post-translational modifications that can occur on their “histone tails”, there are a vast number of possibilities when it comes to the exact identiity of a nucleosome and its function. Recently, some of these variants and modifications have been found to associate with cancers. Furthermore, just the total amount of nucleosomes detected can also indicate a disease state or correlate with efficacy of therapy.

    Recently, “liquid biopsies”, or blood draws from which you can examine biomarkers, have taken hold as an easy, less invasive method to monitor disease state and treatment efficacy. Nucleosomes, histones, and DNA can be shed into the blood during cancer due to high cellular turnover or in response to chemotherapy. Free-floating DNA (cfDNA and ctDNA) is an attractive biomarker, but has shown to be less stable than nucleosomes. Additionally, next generation sequencing of DNA is cumbersome and costly compared to a high-throughput ELISA assay to detect intact nucleosomes. Nucleosome-specific detection assays represent a new generation of biomarker tests: easy, fast, cost-effective, and compatible with stable proteins in liquid biopsy samples.

Histone H3 Phosphorylation Antibody Panel Pack

C10014
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Acetylation Antibody Panel Pack I

C10010
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Acetylation Antibody Panel Pack II

C10011
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack I – Active Genes

C10000
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack I – Repression Genes

C10001
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Active Genes

C10002
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Repression Genes

C10003
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack III – Active Genes

C10004
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Tertiapin-Q

B5090-1 1 mg
EUR 656.4

NU 9056

A4492-10 10 mg
EUR 340.8
Description: Selective KAT5 (Tip60) histone acetyltransferase inhibitor (IC50 values are 100 ?M for KAT5, p300, pCAF and GCN5, respectively). Inhibits protein acetylation in prostate cancer cell lines and blocks DNA damage response.

NU 1025

A4530-25 25 mg
EUR 528
Description: Novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP). Ki and IC50 values are 48 and 400 nM respectively.

NU 1025

A4530-5 5 mg
EUR 216
Description: Novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP). Ki and IC50 values are 48 and 400 nM respectively.

NU 1025

A4530-50 50 mg
EUR 945.6
Description: Novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP). Ki and IC50 values are 48 and 400 nM respectively.

NU 7026

A8649-10 10 mg
EUR 164.4
Description: NU 7026 is a selective inhibitor of  DNA-PK with IC50 value of 0.23 ?M [1].

NU 7026

A8649-50 50 mg
EUR 505.2
Description: NU 7026 is a selective inhibitor of  DNA-PK with IC50 value of 0.23 ?M [1].

Nu 6027

A1986-10 10 mg
EUR 211.2
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

Nu 6027

A1986-5 5 mg
EUR 154.8
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

Nu 6027

A1986-5.1 10 mM (in 1mL DMSO)
EUR 157.2
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

Nu 6027

A1986-50 50 mg
EUR 656.4
Description: NU6027 is a potent inhibitor of cellular ATR activity with the IC50 of 6.7 ?M.The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a crucial role in the stalled replication forks signalling and DNA damage to cell cycle checkpoints and DNA repair.

NU 7026

HY-15719 10mM/1mL
EUR 151.2

NU-7441

B1117-25 each
EUR 652.8

NU-7441

B1117-5 each
EUR 210

NU-7026

B2522-25 each
EUR 652.8

NU-7026

B2522-5 each
EUR 210

NU 1025

2351-5 each
EUR 314.4

NU 7026

27312 50 mg
EUR 525
Description: PARP3 Inhibitor

Q-VD-OPh

1170-1 each
EUR 280.8

anti-PKC nu (Protein kinase C nu)

LF-PA0073 100 ul
EUR 400.8
Description: Rabbit polyclonal to PKC nu (Protein kinase C nu)

Histone H3

79055-1 100 µg
EUR 70
Description: Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 1-136(end), MW = 15 kDa, expressed in an E. coli expression system.

LabTek 1 Well Permanox Slide RUO

177410K PK16
EUR 299.82

Q-VD(OMe)-OPh

A8165-1 1 mg
EUR 212.4
Description: Q-VD-OPh (quinolyl-valyl-O-methylaspartyl-[-2,6-difluorophenoxy]-methyl ketone) is a broad spectrum caspase inhibitor,  provides a cost effective, non toxic, and highly specific means of apoptotic inhibition and provides new insight into the design of new inhibitors.

Q-VD-OPh hydrate

A1901-1 1 mg
EUR 212.4
Description: The broad spectrum caspase inhibitor, QVD-OPh, provides a cost effective, non toxic, and highly speci?c means of apoptotic inhibition and provides new insight into the design of new inhibitors1.

EZSolution? Q-VD-OPh

1173-1 each
EUR 248.4

EZSolution? Q-DEVD-OPh

1178-1 each
EUR 326.4

Q-VD(OMe)-OPh

27640-1 5 mg
EUR 380
Description: Q-VD-(OMe)-Ph is a second-generation capase inhibitor that is highly potent, brain and cell permeable, irreversible and nontoxic. Q-VD-(OMe)-Ph can inhibit caspase-3 ( IC50=25 nm), caspase-1 (IC50=50 nM), caspase-8 (IC50=100 nM) and caspase-9 (IC50=430 nM).

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit 

P-3001
  • EUR 1050.06
  • EUR 424.60
  • EUR 712.80
  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K4) 

P-3002
  • EUR 1067.46
  • EUR 437.80
  • EUR 724.90
  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K9) 

P-3003
  • EUR 1067.46
  • EUR 437.80
  • EUR 724.90
  • 96 Assays
  • 48 Assays
  • 96 Assays

Protein Kinase C Nu Type (PKC nu) Antibody

abx033122-400ul 400 ul
EUR 627.6

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