Monoclonal antibody from mouse specific for BAFF-R

The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for common: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Subtle is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to supply a blue shade product that turned yellow after along with acidic cease reply. The density of yellow is proportional to the Human BAFF-R quantity of pattern captured in plate.

Key decisions and particulars

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Pattern kind: Cell customized supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay kind: Sandwich (quantitative)
  • Reacts with: Human
  • Product set up

    Human BAFF-R ELISA Instruments
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Pattern n Point out SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Inter-assay
    Pattern n Point out SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%
    bio human baff r
    bio human baff r

    Pattern kind

    Cell customized supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay kind

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay dimension

    Loads of steps commonplace assay
  • Species reactivity

    Reacts with: Human

Product overview

The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell customized supernatants, cell lysates, serum and plasma (heparin, EDTA).

 

Notes

BAFF-R (moreover generally known as Tumor necrosis problem receptor superfamily member 13C or B-cell-activating problem receptor)  is a protein that in people is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating problem (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Platform

Pre-coated microplate (12 x Eight appropriately strips)

Human B cell activating factor of TNF family(BAFF)ELISA Kit

QY-E04538 96T
EUR 433.2

BAFF-R, Human

HY-P7130 10ug
EUR 183.6

Rat B-Cell Activating Factor(BAFF)ELISA Kit

QY-E10385 96T
EUR 433.2

Mouse B-Cell Activating Factor(BAFF)ELISA Kit

QY-E20186 96T
EUR 433.2

Human BAFF-R(CD268)

90103-A 10 µg
EUR 130
Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli.

Human BAFF-R(CD268)

90103-B 50 µg
EUR 205
Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli.

BAFF-R(human):Fc(human)

BC-323 50 ug
EUR 667

[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human)

030-51 100 μg
EUR 177.12

BAFF Receptor Antibody / BAFF-R

RQ4381 100 ug
EUR 356.15
Description: Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.

Baff receptor Antibody / Baff-r

RQ4338 100 ug
EUR 356.15
Description: Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.

[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human) - Antibody

H-030-51 50 μl
EUR 604.8

Recombinant Human BAFF-R (TNFRSF13C)

Z100015 50 µg
EUR 105
Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.

Recombinant Human BAFF-R (TNFRSF13C)

Z100017 100 µg
EUR 215
Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.

Recombinant Human BAFF-R (TNFRSF13C)

Z100019 1.0 mg
EUR 1060
Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.

[Cys0]-BAFF-R (108-129) (Human)

030-52 100 μg
EUR 177.12

Human BAFF-R Protein, mFc Tag

E33P100044 10 μg
EUR 225

Human BAFF-R Protein, hFc Tag

E33P100503 10 μg
EUR 225

BAFF-R Antibody

3578R-100 each
EUR 379.2

BAFF-R Antibody

3578R-30T each
EUR 175.2

BAFF-R Antibody

R30462 100 ug
EUR 356.15
Description: Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFF-R, is a protein in humans is encoded by the TNFRSF13C gene. The gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.

[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human) - Purified IgG Antibody

G-030-51 200 μg
EUR 604.8

Bio Bucket™ collapsible laboratory ice bucket, 5 Liter, 1/each

B8300 1/pack
EUR 54
Description: Bio Bucket

Human tenascin-R(TN-R)ELISA Kit

QY-E00282 96T
EUR 433.2

Biotinylated Recombinant Human TNFRSF13C/BAFF-R/CD268 Protein

RP02582 1000μg
EUR 422.5

BAFF-R Blocking Peptide

3578RBP-50 each
EUR 183.6

BAFF-R Polyclonal Antibody

3578-100 each
EUR 379.2

Polyclonal BAFF-R Antibody

APR15125G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications:

BAFF-R Recombinant Protein

90-237 50 ug
EUR 600.9
Description: BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers.

PE anti-human CD268 (BAFF-R)

E16FHP268-025 25 tests
EUR 476.67
Description: Available in various conjugation types.

PE anti-human CD268 (BAFF-R)

E16FHP268-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

APC anti-human CD268 (BAFF-R)

E16FHA268-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

BAFF, Human

E34M050H 5 μg
EUR 155

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